Translator Disclaimer
1 November 2004 CHARACTERIZATION OF A SPONTANEOUSLY POLARIZING HT-29 CELL LINE, HT-29/cl.f8
Author Affiliations +
Abstract

A cloned cell line that spontaneously polarizes in standard glucose-containing media was derived from a single cell of the adenocarcinoma cell line HT-29. The cloned line, designated HT-29/cl.f8, has remained stable over 2 yr in culture, maintained high transepithelial resistance (300 ohm cm2 or higher), and correctly sorted influenza virus and vesicular stomatitis virus to apical or basolateral domains, respectively. The newly cloned cells also displayed apical microvilli, tight junctions, and desmosomes, the morphological characteristics of mature epithelia. The cloned HT-29/cl.f8 cells function as epithelial enterocytes as shown by the apical expression of intestinal alkaline phosphatase, the expression of vimentin and cytokeratin, and lack of expression of mucin. We propose that the newly cloned HT-29/cl.f8 cells offer a viable alternative for studies of enterocyte function that will readily yield interpretable data not complicated by cell alterations due to the presence of drugs or chemicals that induce differentiation.

DEANNE M. MITCHELL and JUDITH M. BALL "CHARACTERIZATION OF A SPONTANEOUSLY POLARIZING HT-29 CELL LINE, HT-29/cl.f8," In Vitro Cellular & Developmental Biology - Animal 40(10), 297-302, (1 November 2004). https://doi.org/10.1290/04100061.1
Received: 28 September 2004; Accepted: 1 October 2004; Published: 1 November 2004
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top