IMAGING BETA CELL DEVELOPMENT IN REAL-TIME USING PANCREATIC EXPLANTS FROM MICE WITH GREEN FLUORESCENT PROTEIN–LABELED PANCREATIC BETA CELLS

SUBHADRA C. GUNAWARDANA; MANAMI HARA; GRAEME I. BELL; W. STEVEN HEAD; MARK A. MAGNUSON; DAVID W. PISTON

doi:10.1290/0412080.1

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SUBHADRA C. GUNAWARDANA,¹ MANAMI HARA,¹ GRAEME I. BELL,¹ W. STEVEN HEAD,¹ MARK A. MAGNUSON,¹ DAVID W. PISTON^1,*

¹Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 735 Lig

^*To whom correspondence should be addressed at dave.piston@vanderbilt.edu

Abstract

We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.

Citation Download Citation

SUBHADRA C. GUNAWARDANA, MANAMI HARA, GRAEME I. BELL, W. STEVEN HEAD, MARK A. MAGNUSON, and DAVID W. PISTON "IMAGING BETA CELL DEVELOPMENT IN REAL-TIME USING PANCREATIC EXPLANTS FROM MICE WITH GREEN FLUORESCENT PROTEIN–LABELED PANCREATIC BETA CELLS," In Vitro Cellular & Developmental Biology - Animal 41(1), 7-11, (1 January 2005). https://doi.org/10.1290/0412080.1

Received: 13 December 2004; Accepted: 9 February 2005; Published: 1 January 2005