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1 May 2005 OPPOSITE EFFECTS OF SEROTONIN AND INTERFERON-α ON THE MEMBRANE POTENTIAL AND FUNCTION OF HUMAN NATURAL KILLER CELLS
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Abstract

In an earlier article, we reported that serotonin (5-hydroxytryptamine, 5-HT) inhibits the natural killer cell (NK) cytotoxicity of human whole blood in a dose-dependent manner and that natural human interferon-α (IFN-α) partially eliminates this effect. Because natural IFN-α might contain factors other than IFN, we repeated these experiments with recombinant human interferon-α (rhIFN-α) and separated blood lymphocytes enriched with NK cells and then demonstrated that IFN really is responsible for this effect. Furthermore, this investigation was carried out to clarify the mechanisms of the action of 5-HT and of rhIFN-α on NK cells. The inhibition of the cytotoxicity was pronounced when 5-HT was added at the onset of the cytotoxic assay, whereas the pretreatment of lymphocytes for 18 h only led to a slight inhibition. Moreover, rhIFN-α applied 1 h before or 1 h after the addition of 5-HT decreased the inhibitory effect of 5-HT. Flow cytometric analysis involving the use of a voltage-sensitive dye, oxonol, revealed that 5-HT depolarized, whereas rhIFN-α hyperpolarized the plasma membrane of the lymphocytes. Thus, it seems likely that the inhibitory effect of 5-HT on the cytotoxicity of peripheral human lymphocytes is due to the depolarization on the plasma membrane of the effector cells and that rhIFN-α antagonizes this ability via its hyperpolarizing activity.

TIBOR OLÁH, IMRE OCSOVSZKI, YVETTE MÁNDI, ROZÁLIA PUSZTAI, MÁRTA BAKAY, and ELISABETH BALINT "OPPOSITE EFFECTS OF SEROTONIN AND INTERFERON-α ON THE MEMBRANE POTENTIAL AND FUNCTION OF HUMAN NATURAL KILLER CELLS," In Vitro Cellular & Developmental Biology - Animal 41(5), (1 May 2005). https://doi.org/10.1290/0407048.1
Received: 15 August 2004; Accepted: 18 January 2005; Published: 1 May 2005
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