Translator Disclaimer
1 May 2005 ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST
Author Affiliations +
Abstract
A cell line, BPE-1, was derived from a parthenogenetic 8-d in vitro-produced bovine blastocyst that produced a cell outgrowth on STO feeder cells. The BPE-1 cells resembled visceral endoderm previously cultured from blastocysts produced by in vitro fertilization (IVF). Analysis of the BPE-1 cells demonstrated that they produced serum proteins and were negative for interferon-tau production (a marker of trophectoderm). Transmission electron microscopy revealed that the cells were a polarized epithelium connected by complex junctions resembling tight junctions in conjunction with desmosomes. Rough endoplasmic reticulum was prominent within the cells as were lipid vacuoles. Immunocytochemistry indicated the BPE-1 cells had robust microtubule networks. These cells have been grown for over 2 yr for multiple passages at 1:10 or 1:20 split ratios on STO feeder cells. The BPE-1 cell line presumably arose from embryonic cells that became diploid soon after parthenogenetic activation and development of the early embryo. However, metaphase spreads prepared at passage 41 indicated that the cell population had a hypodiploid (2n = 60) unimodal chromosome content with a mode of 53 and a median and mean of 52. The cell line will be of interest for functional comparisons with bovine endoderm cell lines derived from IVF and nuclear transfer embryos.
NEIL C. TALBOT, THOMAS J. CAPERNA, ANNE M. POWELL, ALAN D. EALY, LE ANN BLOMBERG and WESLEY M. GARRETT "ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST," In Vitro Cellular & Developmental Biology - Animal 41(5), (1 May 2005). https://doi.org/10.1290/040901.1
Received: 16 September 2004; Accepted: 24 March 2005; Published: 1 May 2005
JOURNAL ARTICLE
12 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top