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1 May 2005 CHARACTERIZATION OF CELL LINES DEVELOPED FROM THE GLASSY-WINGED SHARPSHOOTER, HOMALODISCA COAGULATA (HEMIPTERA: CICADELLIDAE)
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Abstract
Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction– amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.
SHIZUO G. KAMITA, ZUNG N. DO, AMAN I. SAMRA, JAMES R. HAGLER and BRUCE D. HAMMOCK "CHARACTERIZATION OF CELL LINES DEVELOPED FROM THE GLASSY-WINGED SHARPSHOOTER, HOMALODISCA COAGULATA (HEMIPTERA: CICADELLIDAE)," In Vitro Cellular & Developmental Biology - Animal 41(5), (1 May 2005). https://doi.org/10.1290/0501002.1
Received: 3 January 2005; Accepted: 11 March 2005; Published: 1 May 2005
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