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1 July 2005 A NOVEL CELL ARRAY TECHNIQUE FOR HIGH-THROUGHPUT, CELL-BASED ANALYSIS
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Abstract

Microarray technology has burgeoned over the past few years from nucleic acid–based arrays to tissue microarrays (TMAs). This study aimed to develop a technique to incorporate cell lines into an array and to demonstrate the usefulness of this technique by performing immunohistochemistry for β-catenin. Cell suspensions were prepared from 23 tumor cell lines. These were fixed in formalin, suspended in agar, and embedded in paraffin to produce a cell block. A “tissue microarrayer” was used to remove triplicate, 0.6 mm-cores from each cell block and to transfer these into a recipient paraffin block at precise coordinates. Immunohistochemistry was used to identify cell lines positive for β-catenin. Cultured cells were successfully incorporated into the microarray, with preservation of cell architecture and even distribution of cells within each core. A total of 18 of 69 cores (26%) were lost in processing. A total of 16 of 23 cell lines were identified as positive for membrane and cytoplasmic β-catenin, and 6 of 23 were negative. Only one cell line was unscorable because of complete core loss. We have developed a “cell microarray” technique for analyzing antigen expression by immunohistochemistry in multiple cell lines in a single experiment. This novel application of microarrays permits high-throughput, cost-efficient analysis, with the potential to rapidly identify markers with potential diagnostic and therapeutic implications in human disease.

A. WATERWORTH, A. HANBY, and V. SPEIRS "A NOVEL CELL ARRAY TECHNIQUE FOR HIGH-THROUGHPUT, CELL-BASED ANALYSIS," In Vitro Cellular & Developmental Biology - Animal 41(7), 185-187, (1 July 2005). https://doi.org/10.1290/0505032.1
Received: 10 May 2005; Accepted: 1 June 2005; Published: 1 July 2005
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