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1 September 2005 INFLUENCE OF SERUM ON ADULT AND FETAL DERMAL FIBROBLAST MIGRATION, ADHESION, AND COLLAGEN EXPRESSION
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Abstract

The wound healing response to injury can be affected by many factors such as cell migration and extracellular matrix elaboration. The objective of this study was to examine the serum- and age-dependent effects on cell migration, adhesion, and collagen expression by skin fibroblasts. Dermal fibroblasts were isolated and plated with and without serum for up to 7 d. Cell migration was determined by quantitative image analysis, adhesion was quantified using a centrifugation assay, and collagen expression was assessed by PCR and immunohistochemical staining. Both adult and fetal fibroblasts migrated significantly faster in serum-containing medium compared to serum-free medium. There was no significant difference in migration between the two cell types in either serum-containing or serum-free medium. There was no significant difference in adhesion in the presence of serum, although there was a greater fraction of adherent fetal skin fibroblasts than adult fibroblasts in serum-free medium. Moreover, the adherent fraction of fetal fibroblasts in serum-free medium was not significantly different from that in serum-containing medium, suggesting that fetal skin fibroblasts possess serum-independent adhesion properties. Collagen mRNA expression was significantly up-regulated in serum-free compared to serum-containing medium for both cell types. With respect to collagen immunohistochemistry, both dermal fibroblast populations exhibited greater type I collagen compared to type III collagen staining. Quantitative assessment of collagen staining indicated significantly enhanced type I collagen secretion in the presence of serum by fetal skin fibroblasts. These findings suggest that intrinsic cellular characteristics may govern the observed differences in adult and fetal wound healing.

HALLIE E. BRINK, SIMONE S. STALLING, and STEVEN B. NICOLL "INFLUENCE OF SERUM ON ADULT AND FETAL DERMAL FIBROBLAST MIGRATION, ADHESION, AND COLLAGEN EXPRESSION," In Vitro Cellular & Developmental Biology - Animal 41(8), (1 September 2005). https://doi.org/10.1290/0503023R.1
Received: 21 March 2005; Accepted: 9 August 2005; Published: 1 September 2005
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