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1 May 2006 TRANSIENT MAINTENANCE IN BIOREACTOR IMPROVES HEALTH OF NEURONAL CELLS
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Abstract

To examine whether a neuronal cell suspension can be held in vitro for a relatively short period without compromising survival rates and functionality, we have set up an experimental protocol planning 24 h of suspension culture in a rotary wall vessel (RWV) bioreactor before plating in a conventional adherent system. Apoptosis measurement and activated caspase-8, -9, and -3 detection have demonstrated that survey of the cells was not affected. The activity of major antioxidant enzymes (AOE), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT), was significantly decreased in RWV-maintained cells. A significant decrease of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) is coupled with a level of activated nuclear factor-κB (NF-κB) protein significantly lower in RVW cells than in the control. On the contrary, the level of IL-6 expression did not change between the test and the control. A significant up-regulation of growth-associated protein-43 (GAP-43), peroxisome proliferator-activated receptor-β/δ (PPARβ/δ), and acyl-CoA synthetase 2 (ACS2) in RWV cells has been detected. We provide the evidence that primary neuronal cells, at an early stage of development, can be maintained in a suspension condition before adherent plating. This experimental environment does not induce detrimental effects but may have an activator role, leading cells to development and maturation in a tridimensional state.

SILVIA DI LORETO, PIERLUIGI SEBASTIANI, ELISABETTA BENEDETTI, VINCENZO ZIMMITTI, VALENTINA CARACCIOLO, FERNANDA AMICARELLI, ANNAMARIA CIMINI, and DOMENICO ADORNO "TRANSIENT MAINTENANCE IN BIOREACTOR IMPROVES HEALTH OF NEURONAL CELLS," In Vitro Cellular & Developmental Biology - Animal 42(5), (1 May 2006). https://doi.org/10.1290/0511077.1
Received: 3 November 2005; Accepted: 6 March 2006; Published: 1 May 2006
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