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18 September 2007 Cytotoxic effect of achatinin h (lectin) from Achatina fulica against a human mammary carcinoma cell line (MCF7)
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Abstract
The hemolymph-derived achatininh (lectin) from Achatina fulica showed a marked cytotoxic effect on MCF7, a human mammary carcinoma cell line. IC50 values as measured by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphe-nyltetrazolium bromide assay for achatininh ranged from 6 to 10 μg/ml in the MCF7 cells. MCF7 cells showed significant morphological changes leading to cell death. The above cell death was observed after 48 h of treatment with 8 μg/ml when compared to untreated cells. Alterations in the tumor marker enzymes, as well as in antioxidant enzymes, were observed after achatininh treatment. The specificity and purity of the achatininh was confirmed by the Western blot assay. Achatininh binding to MCF7 cells was detected by anti-achatininh, and visualization of the achatininh binding sites on confluent MCF7 cells was confirmed by flourescein isothiocyanate conjugated secondary antibody. MCF7-treated cells fluoresced, indicating the presence of achatininh binding sites. Fluorescence-activated cell sorting analysis of the cell cycle showed a significant increase in S-phase in MCF7 cells after 48 h of achatininh treatment. The cells were arrested in G2/M phase of the cell cycle after 48 h with significant changes in cell viability. Cellular damage was confirmed by agarose gel electrophoresis with the characteristic appearance of a DNA streak in treated MCF7 cells indicating the ongoing apoptosis.
Indra Dharmu, N. Ramamurty, Ramalingam Kannan and Mary Babu "Cytotoxic effect of achatinin h (lectin) from Achatina fulica against a human mammary carcinoma cell line (MCF7)," In Vitro Cellular & Developmental Biology - Animal 43(8), (18 September 2007). https://doi.org/10.1007/s11626-007-9055-z
Received: 26 March 2007; Accepted: 26 July 2007; Published: 18 September 2007
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