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22 November 2007 Neoplastic transformation and induction of H ,K -adenosine triphosphatase by N-methyl-N′-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line
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Abstract
N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) induces gastric cancer in animal models. We established an MNNG-induced mutant of the rat murine RGM-1 gastric epithelial cell line, which we named RGK-1, that could be used as an in vitro model of gastric cancer. This cell line showed signs of neoplasia and transformation, in that it lost contact inhibition and formed tumors in nude mice. The mutant cells also expressed parietal cell-specific H ,K -adenosine triphosphatase (H ,K -ATPase), which parent RGM-1 did not. The results suggested that parent RGM-1 cells were gastric progenitor cells. This mutant RGK-1 cell line will contribute to future investigation on gastric carcinogenesis and to the development of other pathophysiologic fields.
Osamu Shimokawa, Hirofumi Matsui, Yumiko Nagano, Tsuyoshi Kaneko, Takeshi Shibahara, Akira Nakahara, Ichinosuke Hyodo, Akinori Yanaka, Hideyuki J. Majima, Yukio Nakamura and Yasushi Matsuzaki "Neoplastic transformation and induction of H ,K -adenosine triphosphatase by N-methyl-N′-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line," In Vitro Cellular & Developmental Biology - Animal 44(1), (22 November 2007). https://doi.org/10.1007/s11626-007-9067-8
Received: 12 July 2007; Accepted: 4 October 2007; Published: 22 November 2007
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