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20 December 2007 TGF-β1 and high levels of glucose do not increase insulin cell proportions in the avian embryonic pancreas
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Abstract
TGF-β1 is thought to decrease the proportion of embryonic pancreatic β-cells with respect to α-cells, whereas glucose is thought to enhance β-cell proportions in rats. However, chick pancreatic cells may respond in a dissimilar way to glucose. Thus, the effect of TGF-β1 on the proportion of β-cells in embryonic chick dorsal pancreatic buds (DPBs) in vitro was tested with short-term exposure to high levels of glucose. Five-day-old chick DPBs were cultured on growth factor-reduced Matrigel, which contains reduced levels of growth factors including TGF-β1, and a variety of culture media with and without high levels of glucose. TGF-β1 reduced the proportion of β-cells, as expected. A similar decrease in the proportion of β-cells occurred in the presence of high levels of glucose.
A. Marais, P. T. Goven-Shiba and B. Kramer "TGF-β1 and high levels of glucose do not increase insulin cell proportions in the avian embryonic pancreas," In Vitro Cellular & Developmental Biology - Animal 44(1), (20 December 2007). https://doi.org/10.1007/s11626-007-9073-x
Received: 22 October 2007; Accepted: 14 November 2007; Published: 20 December 2007
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