TGF-β1 is thought to decrease the proportion of embryonic pancreatic β-cells with respect to α-cells, whereas glucose is thought to enhance β-cell proportions in rats. However, chick pancreatic cells may respond in a dissimilar way to glucose. Thus, the effect of TGF-β1 on the proportion of β-cells in embryonic chick dorsal pancreatic buds (DPBs) in vitro was tested with short-term exposure to high levels of glucose. Five-day-old chick DPBs were cultured on growth factor-reduced Matrigel, which contains reduced levels of growth factors including TGF-β1, and a variety of culture media with and without high levels of glucose. TGF-β1 reduced the proportion of β-cells, as expected. A similar decrease in the proportion of β-cells occurred in the presence of high levels of glucose.
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20 December 2007
TGF-β1 and high levels of glucose do not increase insulin cell proportions in the avian embryonic pancreas
A. Marais,
P. T. Goven-Shiba,
B. Kramer
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In Vitro Cellular & Developmental Biology - Animal
Vol. 44 • No. 1
January 2008
Vol. 44 • No. 1
January 2008
Glucose
Pancreatic β-cells
TGF-β1