Translator Disclaimer
21 August 2008 The frequency, growth kinetics, and osteogenic/adipogenic differentiation properties of canine bone marrow stromal cells
Author Affiliations +
Abstract

Bone marrow stromal cells (BMSCs) have gained considerable attention as a potential source for cell transplantation therapies for a variety of diseases due to their accessibility, proliferative capacity, and multilineage differentiation properties. Canine BMSCs have been shown to contribute to regeneration of osseous tissues, but knowledge about their biology is currently limited. In the present study, we investigated the frequency of adult canine BMSCs in bone marrow, morphological features, growth kinetics, and osteogenic as well as adipogenic differentiation properties in vitro. Our data suggest that adult canine bone marrow contains approximately one BMSC in every 2.38×104 bone marrow mononucleated cells (0.0042± 0.0019%, n=5). Primary BMSC cultures consisted of morphologically heterogeneous adherent cell populations from which spindle-shaped cells grew and became the predominant cell type. Growth kinetics patterns were dependent on the initial cell seeding densities, resulting in the highest fold increase at lower cell density. In the presence of osteogenic and adipogenic inducers, primary BMSCs underwent morphological and phenotypic changes characteristic of osteogenic and adipogenic differentiation, respectively. This study provides insights into basic characterization of adult canine BMSCs.

Hiroaki Kamishina, James P. Farese, Joshua A. Storm, Jennifer A. Cheeseman, and Roger M. Clemmons "The frequency, growth kinetics, and osteogenic/adipogenic differentiation properties of canine bone marrow stromal cells," In Vitro Cellular & Developmental Biology - Animal 44(10), 472-479, (21 August 2008). https://doi.org/10.1007/s11626-008-9137-6
Received: 15 February 2008; Accepted: 16 July 2008; Published: 21 August 2008
JOURNAL ARTICLE
8 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top