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5 February 2008 Establishment and characterization of OS 99-1, a cell line derived from a highly aggressive primary human osteosarcoma
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Abstract
Osteosarcoma is the most common form of primary bone cancer. In this study, we established a human osteosarcoma cell line (OS 99-1) from a highly aggressive primary tumor. G-banding karyotype analysis demonstrated a large number of clonal abnormalities, as well as extensive intercellular heterogeneity. Through the use of immunologic, molecular, and biochemical analyses, we characterized protein and gene expression profiles confirming the osteogenic nature of the cells. Further evaluation indicated that OS 99-1 cells maintain the capacity to differentiate in an in vitro mineralization assay as well as form tumors in the in vivo chicken embryo model. This cell line provides a useful tool to investigate the molecular mechanisms contributing to osteosarcoma and may have the potential to serve as a culture system for studies involving bone physiology.
Jennifer M. Gillette, C. Parker Gibbs and Sheila M. Nielsen-Preiss "Establishment and characterization of OS 99-1, a cell line derived from a highly aggressive primary human osteosarcoma," In Vitro Cellular & Developmental Biology - Animal 44(3), (5 February 2008). https://doi.org/10.1007/s11626-007-9075-8
Received: 2 August 2007; Accepted: 20 November 2007; Published: 5 February 2008
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