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23 February 2008 An improved protocol for primary culture of cardiomyocyte from neonatal mice
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Abstract
The primary culture of neonatal mice cardiomyocyte model enables researchers to study and understand the morphological, biochemical, and electrophysiological characteristics of the heart, besides being a valuable tool for pharmacological and toxicological studies. Because cardiomyocytes do not proliferate after birth, primary myocardial culture is recalcitrant. The present study describes an improved method for rapid isolation of cardiomyocytes from neonatal mice, as well as the maintenance and propagation of such cultures for the long term. Immunocytochemical and gene expression data also confirmed the presence of several cardiac markers in the beating cells during the long-term culture condition used in this protocol. The whole culture process can be effectively shortened by reducing the enzyme digestion period and the cardiomyocyte enrichment step.
P. Sreejit, Suresh Kumar and Rama S. Verma "An improved protocol for primary culture of cardiomyocyte from neonatal mice," In Vitro Cellular & Developmental Biology - Animal 44(3), (23 February 2008). https://doi.org/10.1007/s11626-007-9079-4
Received: 13 September 2007; Accepted: 11 December 2007; Published: 23 February 2008
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