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1 May 2004 IN VITRO PROPAGATION OF ZINGIBER PETIOLATUM (HOLTTUM) I. THEILADE, A RARE ZINGIBERACEOUS PLANT FROM THAILAND
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Abstract

We describe an in vitro propagation protocol for Zingiber petiolatum (Holttum) I. Theilade, a rare species from the southern part of Thailand. Fruits were surface-sterilized and seeds germinated on Murashige and Skoog medium (MS) medium supplemented with 3% sucrose. Three-month-old seedlings were used as initial plant material for in vitro propagation. Terminal buds of the plants were inoculated on MS medium containing 6-benzylaminopurine (BA; 2.2–35.5 μM) alone or in combination with 1-naphthaleneacetic acid (0.5 μM). Eight weeks after inoculation, the cultures were transferred to MS medium without plant growth regulators for 4 wk. The cultures transferred from MS medium with 17.8 μM BA revealed the highest shoot induction rate of 6.1 ± 0.7 shoots per explant. Rooting was spontaneously achieved in MS medium without plant growth regulators. Rooted plants were successfully transplanted to soil.

SOMPOP PRATHANTURARUG, DUANGPORN ANGSUMALEE, NUTTINEE PONGSIRI, SUPARAT SUWACHARANGOON, and THAYA JENJITTIKUL "IN VITRO PROPAGATION OF ZINGIBER PETIOLATUM (HOLTTUM) I. THEILADE, A RARE ZINGIBERACEOUS PLANT FROM THAILAND," In Vitro Cellular and Developmental Biology - Plant 40(3), 317-320, (1 May 2004). https://doi.org/10.1079/IVP2003505
Received: 16 May 2003; Accepted: 1 September 2003; Published: 1 May 2004
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