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1 May 2005 ORGANOGENESIS, EMBRYOGENESIS, AND SYNTHETIC SEED PRODUCTION IN ARNEBIA EUCHROMA – A CRITICALLY ENDANGERED MEDICINAL PLANT OF THE HIMALAYA
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Abstract

This is the first report of simultaneous organogenesis and somatic embryogenesis in Arnebia euchroma, a highly valued, critically endangered medicinal plant of the Himalaya. Root-derived callus showed only rhizogenesis, whereas leaf-derived callus showed simultaneous organogenesis and somatic embryogenesis. Organogenesis was optimal (12.2 shoots per culture) in 1 μM indole-3-butyric acid combined with 2.5 μM 6-benzyladenine and induction of somatic embryogenesis (16.3 embryos per culture) occurred in 2.5 μM indole-3-butyric acid combined with 2.5 μM 6-benzyladenine. Shoots rooted (100%) best in half-strength Murashige and Skoog (MS) medium supplemented with 2.0 μM indole-3-butyric acid. Early cotyledonary-stage embryos encapsulated with 3% sodium alginate and calcium nitrate (100 mM for 25 min) showed 60.6% germination in MS medium. Rooted shoots transferred to a mixture of sterile soil, sand, and peat (1:1:1 by volume) showed 72% survival ex vitro. Application of these protocols would be helpful in reducing pressure in natural populations, in genetic transformation studies, and in long-term storage of elite genotypes through synthetic seed production.

SUMIT MANJKHOLA, UPPEANDRA DHAR, and MEENA JOSHI "ORGANOGENESIS, EMBRYOGENESIS, AND SYNTHETIC SEED PRODUCTION IN ARNEBIA EUCHROMA – A CRITICALLY ENDANGERED MEDICINAL PLANT OF THE HIMALAYA," In Vitro Cellular and Developmental Biology - Plant 41(3), (1 May 2005). https://doi.org/10.1079/IVP2004612
Received: 14 May 2004; Accepted: 1 October 2004; Published: 1 May 2005
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