To pursue the causal factors of ploidy variation in tissue culture of asparagus (Asparagus officinalis L.), ploidy status of stem explants and the calluses induced from these explants were examined using flow cytometry (FCM). The frequency of higher ploidy (>8C) cells in the stem explants increased with increasing distance from the shoot apex. Calluses derived from sections far from the apex also showed higher ploidy distributions, and also higher ploidy (16C) in general than was observed in the explant tissue. Detailed studies using FCM and charge-coupled device (CCD)-equipped fluorescence microscopy showed that 16C cells appeared in the explant tissues within 1 wk of culture, possibly induced by the plant growth regulators (PGRs), especially 1-naphthaleneacetic acid (NAA), in the culture medium.
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1 January 2006
PLOIDY DISTRIBUTION IN THE EXPLANT TISSUE AND THE CALLUSES INDUCED DURING THE INITIAL STAGE OF INTERNODE SEGMENT CULTURE OF ASPARAGUS OFFICINALIS L.
KEI-ICHIRO MISHIBA,
KEI-ICHI TAWADA,
MASAHIRO MII
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In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 1
January 2006
Vol. 42 • No. 1
January 2006
Asparagus officinalis L
Callus
endoreduplication
flow cytometry
polysomaty
Somaclonal variation