A micropropagation protocol for Pouteria lucuma R. and Pav. var. La Molina was developed. Shoots from zygotic embryos with a portion of endosperm were established in vitro on Murashige and Skoog (MS) medium with 0.47 μM kinetin (Kin) and 0.54 μM naphthaleneacetic acid (NAA). Multiplication of shoots was accomplished using subapical shoots. The best axillary-shoot production was observed on MS basal medium with 2.2 μM benzyladenine (BA), 0.5 μM NAA, 1.4 μM gibberellic acid (GA₃), and 40 mg l⁻¹ adenine sulfate, with the development of up to three axillary shoots per subapical shoot. One hundred percent rooting was obtained from shoots grown for 4 wk on MS medium with 246 μM indole-3-butyric acid under light conditions. Eighty percent of the microplantlets survived after acclimatization when transplanted to a substrate previously enriched with beneficial soil bacteria. This study describes, for the first time, arbuscular mycorrhizal (AM) colonization of this species. Inoculation with AM fungi improved growth and development of lucumo plants and induced changes to the root morphology.