This study reports a protocol for successful micropropagation of Penthorum chinense using nodal explants on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) or kinetin (Kn). The presence of BA promoted a higher rate of shoot multiplication than Kn. Maximum multiple shoot formation was observed in 59.2% of nodal explants cultured on MS medium supplemented with 2.0 mg l−1 BA after 6 wk. After subculture for 4 wk, the maximum number of shoots (6.4) was obtained on a medium with 2.0 mg l−1 BA, but shoots were too short and not suitable for micropropagation. The taller shoots that regenerated in the presence of lower BA concentration (1.0 mg l−1) were selected for root induction study. Most shoots (98.8%) rooted in the presence of 0.5 mg l−1 indole-3-acetic acid after 3 wk, with each shoot forming an average of 10.0 roots. Plantlets were transferred to soil and successfully acclimatized.
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16 March 2007
Micropropagation of Penthorum chinense through axillary bud
H. Cao,
J. Yang,
Z. S. Peng,
C. Y. Kang,
D. C. Chen,
Z. C. Gong,
X. Tan
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In Vitro Cellular and Developmental Biology - Plant
Vol. 43 • No. 2
March 2007
Vol. 43 • No. 2
March 2007
In vitro propagation
Medicinal plant
Penthorum chinense