The in vivo activities of arginine and ornithine decarboxylases, key enzymes in the biosynthesis of putrescine and thus polyamines, were measured in three different cell lines of carrot (Daucus carota) during growth and somatic embryogenesis. The activities of these two enzymes differed in the different cell lines in the presence of various levels of auxin (2,4 dichlorophenoxy acetic acid), but was highest during periods of active cell division. During somatic embryo development, the activities of both enzymes were highest during globular stage formation. Thus, both enzymes were found to be active during growth and somatic embryogenesis and could contribute to polyamine biosynthesis.
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19 September 2007
Arginine and ornithine decarboxylases in embryogenic and non-embryogenic carrot cell suspensions
Natalia Loukanina,
Trevor A. Thorpe
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In Vitro Cellular and Developmental Biology - Plant
Vol. 44 • No. 1
January 2008
Vol. 44 • No. 1
January 2008
Cell culture growth
Daucus carota
Polyamine biosynthesis
Putrescine formation
somatic embryogenesis