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2 October 2008 In vitro induction and identification of autotetraploids of Dioscorea zingiberensis
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Abstract

Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number of autotetraploid plants. The induction rate reached as high as 36.7% of treated calli. More than 50 lines of autotetraploid plants were obtained. All tetraploid plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination of morphological characteristics and for chemical assays. Six elite lines have been selected for further selection and breeding into new varieties for commercial production.

Huang Heping, Gao Shanlin, Chen Lanlan, and Jiao Xiaoke "In vitro induction and identification of autotetraploids of Dioscorea zingiberensis," In Vitro Cellular and Developmental Biology - Plant 44(5), 448-455, (2 October 2008). https://doi.org/10.1007/s11627-008-9177-3
Received: 24 April 2008; Accepted: 10 September 2008; Published: 2 October 2008
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