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1 September 2005 Identification and Initial Characterization of an Adenovirus Associated With Fatal Hepatic and Lymphoid Necrosis in a Meyer's Parrot (Poicephalus meyeri)
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Abstract
A juvenile Meyer's parrot (Poicephalus meyeri) was presented for acute depression and crop stasis and died. On histopathologic examination, intranuclear inclusion bodies were seen in the liver, spleen, proventriculus, and intestinal mucosa. DNA in situ hybridization of tissues was positive for adenovirus and negative for herpesvirus, polyomavirus, and circovirus. Degenerate (polymerase chain reaction [PCR]) primers targeting a conserved region of adenovirus DNA–dependent DNA polymerase were used to amplify and sequence a product from paraffin-embedded tissue. This is the first sequence information available for a psittacine adenovirus. Phylogenetic and comparative sequence analysis indicated that this virus is a member of the genus Aviadenovirus and is here termed Meyer's parrot adenovirus 1. Consensus nested PCR and sequencing can be used to rapidly identify novel adenoviruses, and DNA in situ hybridization may be used for rapid localization of novel viruses in tissues. Identification of adenoviral types and species will provide useful diagnostic, prognostic, and epidemiologic information for clinicians.
James F. X. Wellehan, April J. Johnson, Kenneth S. Latimer, Karyn Bischoff, Maud Lafortune and Elliot R. Jacobson "Identification and Initial Characterization of an Adenovirus Associated With Fatal Hepatic and Lymphoid Necrosis in a Meyer's Parrot (Poicephalus meyeri)," Journal of Avian Medicine and Surgery 19(3), (1 September 2005). https://doi.org/10.1647/2004-003.1
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