Translator Disclaimer
1 October 2011 Molecular-Based Method To Distinguish the Sibling Species Hemigrapsus penicillatus and Hemigrapsus takanoi (Decapoda: Brachyura: Varunidae)
Author Affiliations +
Abstract

In this study, we developed a simple and rapid restriction fragment length polymorphism (RFLP) method to distinguish the sibling species Hemigrapsus penicillatus and H. takanoi based on interspecific base substitutions within the 16S rRNA region of mitochondrial DNA. Sequencing and alignment of partial sequences revealed two haplotypes for H. penicillatus and one for H. takanoi, and five species-specific base substitutions. Digestion of the PCR products using the restriction endonuclease Dde I produced a banding pattern that was consistent with species identification based on morphological and pigmentation patterns. The sequence for H. takanoi collected in Japan showed high homology with that reported for specimens collected in Europe. This method could be useful for identification of these two species by researchers without specific taxonomic knowledge.

Izumi Yamasaki, Wataru Doi, Winda Mercedes Mingkid, Masashi Yokota, Carlos Augusto Strüssmann, and Seiichi Watanabe "Molecular-Based Method To Distinguish the Sibling Species Hemigrapsus penicillatus and Hemigrapsus takanoi (Decapoda: Brachyura: Varunidae)," Journal of Crustacean Biology 31(4), (1 October 2011). https://doi.org/10.1651/10-3366.1
Received: 8 July 2010; Accepted: 1 March 2011; Published: 1 October 2011
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top