Field and laboratory colonies of Helicoverpa zea (Boddie) and Heliothis virescens (F.) were exposed to Cry1Ac and Cry2Ab2 toxins to compare different mortality and mortality stunting responses. Lethal concentrations for mortality (LC50s), molt inhibitory concentrations for mortality surviving L1 larvae (MIC50s), and inhibitory concentrations for mortality surviving L1 and L2 larvae (IC50s) were established to describe the different responses across a range of test concentrations. MIC50S and IC50s at 7 d significantly correlated to LC50s at 7 d, but MIC50s were more closely correlated with MIC50s than IC50s. Experiments conducted to determine the fate of survivors (larval stage L1, L2, or L3 larvae) after 7 d of exposure of Bt toxin and transfer to untreated or Bt-treated diet revealed that significant numbers of stunted larvae recovered and survived to pupation. Percentage of survival was significantly higher for larvae fed untreated diet after 7 d on Bt-treated diet, but a few survivors also were found when larvae were fed only Bt diet. Survival to pupation of larvae classified as L1 at 7 d was significantly lower than those for L2 or L3 larvae at 7 d, indicating that many stunted larvae suffer chronic effects of the initial exposure to Bt and fail to reach the next life stage. Pupation of larvae classified as L1 at 7d was reduced and delayed in comparison with those for L2 and L3 larvae at 7d. Collectively, these data suggest that MIC50 estimates at 7 d more accurately project total larval mortality from Bt exposure than do LC50 or IC50 at 7 d.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 102 • No. 5