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1 December 2009 A Modified Excised Leaf Disc Method to Estimate the Toxicity of Slow- and Fast-Acting Reduced-Risk Acaricides to Mites
Noubar J. Bostanian, Serge Beudjekian, Erin McGregor, Gaétan Racette
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Abstract

The “modified excised leaf disc method” is based on leaf discs that fit tightly the bottom halves of 50-mm petri dishes. The bottom half of each petri dish is covered with wet cotton wool to prolong leaf freshness. The side wall of each bottom half has a small hole to allow the petiole of the leaf disc to protrude outside the petri dish. The top half of each petri dish has a 28-mm (diameter) window. For phytophagous mites the window is covered with a 40-µm mesh Pecap polyester screen. Using this method it was possible to estimate the LC50 value of bifenazate to be 0.00413 g (AI) /liter and the LC50 value of spirodiclofen to be 0.40050 g (AI)/liter to the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). The method reduces losses due to escapees and allows observations to be made as long as 9 d after treatment.

Noubar J. Bostanian, Serge Beudjekian, Erin McGregor, and Gaétan Racette "A Modified Excised Leaf Disc Method to Estimate the Toxicity of Slow- and Fast-Acting Reduced-Risk Acaricides to Mites," Journal of Economic Entomology 102(6), 2084-2089, (1 December 2009). https://doi.org/10.1603/029.102.0610
Received: 9 April 2009; Accepted: 1 August 2009; Published: 1 December 2009
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KEYWORDS
bioassays
laboratory method
lethal concentration
Tetranychus urticae
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