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1 June 2012 Differentially Expressed Genes of Coptotermes formosanus (Isoptera: Rhinotermitidae) Challenged by Chemical Insecticides
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Abstract

Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae) termites are harmful social insects to wood constructions. The current control methods heavily depend on the chemical insecticides with increasing resistance. Analysis of the differentially expressed genes mediated by chemical insecticides will contribute to the understanding of the termite resistance to chemicals and to the establishment of alternative control measures. In the present article, a full-length cDNA library was constructed from the termites induced by a mixture of commonly used insecticides (0.01% sulfluramid and 0.01% triflumuron) for 24 h, by using the RNA ligase-mediated Rapid Amplification cDNA End method. Fifty-eight differentially expressed clones were obtained by polymerase chain reaction and confirmed by dot-blot hybridization. Forty-six known sequences were obtained, which clustered into 33 unique sequences grouped in 6 contigs and 27 singlets. Sixty-seven percent (22) of the sequences had counterpart genes from other organisms, whereas 33% (11) were undescribed. A Gene Ontology analysis classified 33 unique sequences into different functional categories. In general, most of the differential expression genes were involved in binding and catalytic activity.

© 2013 Entomological Society of America
Yi Zhang, Yuanyuan Zhao, Xuehong Qiu, and Richou Han "Differentially Expressed Genes of Coptotermes formosanus (Isoptera: Rhinotermitidae) Challenged by Chemical Insecticides," Journal of Economic Entomology 106(4), 1863-1870, (1 June 2012). https://doi.org/10.1603/EC13057
Received: 29 January 2013; Accepted: 1 May 2013; Published: 1 June 2012
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