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1 February 2014 Use of Spent Mushroom Substrate for Production of Bacillus thuringiensis by Solid-State Fermentation
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Abstract

The aim of this study was to explore a cost-effective method for the mass production of Bacillus thuringiensis (Bt) by solid-state fermentation. As a locally available agroindustrial byproduct, spent mushroom substrate (SMS) was used as raw material for Bt cultivation, and four combinations of SMS-based media were designed. Fermentation conditions were optimized on the best medium and the optimal conditions were determined as follows: temperature 32°C, initial pH value 6, moisture content 50%, the ratio of sieved material to initial material 1:3, and inoculum volume 0.5 ml. Large scale production of B. thuringiensis subsp. israelensis (Bti) LLP29 was conducted on the optimal medium at optimal conditions. High toxicity (1,487 international toxic units/milligram) and long larvicidal persistence of the product were observed in the study, which illustrated that SMS-based solid-state fermentation medium was efficient and economical for large scale industrial production of Bt-based biopesticides. The cost of production of 1 kg of Bt was approximately US$0.075.

© 2014 Entomological Society of America
Songqing Wu, Yanjiao Lan, Dongmei Huang, Yan Peng, Zhipeng Huang, Lei Xu, Ivan Gelbič, Rebeca Carballar-Lejarazu, Xiong Guan, Lingling Zhang, and Shuangquan Zou "Use of Spent Mushroom Substrate for Production of Bacillus thuringiensis by Solid-State Fermentation," Journal of Economic Entomology 107(1), 137-143, (1 February 2014). https://doi.org/10.1603/EC13276
Received: 9 June 2013; Accepted: 1 December 2013; Published: 1 February 2014
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