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1 June 2014 Molecular Diagnostics of Drosophila suzukii (Diptera: Drosophilidae) using PCR-RFLP
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Abstract

The invasive spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), has become a serious pest in the United States. Identification of immature and poorly preserved specimens can be difficult. A molecular diagnostic method for distinguishing D. suzukii from other Drosophila spp. associated with fruit in the United States was developed. A 709-bp region of the mitochondrial DNA cytochrome oxidase I gene was amplified from D. suzukii collections in the United States and compared with sequences of other Drosophila taxa from GenBank. Based on DNA sequence polymorphisms, a polymerase chain reaction—restriction fragment length polymorphism analysis using the restriction enzyme Msp-I was found to differentiate D. suzukii from other Drosophila spp. in the United States. This technique can identify field-collected specimens from various sources and specimens regardless of life stage. This molecular diagnostic method will be useful for monitoring the spread of this economically important invasive insect.

© 2014 Entomological Society of America
Sh S. Kim, A. D. Tripodi, D. T. Johnson, and A. L. Szalanski "Molecular Diagnostics of Drosophila suzukii (Diptera: Drosophilidae) using PCR-RFLP," Journal of Economic Entomology 107(3), 1292-1294, (1 June 2014). https://doi.org/10.1603/EC13389
Received: 9 September 2013; Accepted: 1 March 2014; Published: 1 June 2014
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