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1 February 2015 Comparison of Two Acute Toxicity Test Methods for the Silkworm (Lepidoptera: Bombycidae)
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Abstract

Silkworm (Bombyx mori) is an economically important insect and an ideal nontarget organism to study the environmental assessment of pesticides. Two acute toxicity test methods, leaf-dipping and quantitative spraying, with five insecticides, were compared for pesticide risk assessment. Based on LC50 values of these insecticides by both methods, the order of toxicity levels of five insecticides tested against silkworm was as follows: emamectin benzoate>lambda-cyhalothrin>imidacloprid>chlorpyrifos>dimethoate. Means of relative confidence interval values for individual insecticides of leaf-dipping and quantitative spraying method were—emamectin benzoate (22.55 and 19.03%), lambda-cyhalothrin (18.03 and 17.71%), imidacloprid (19.21 and 16.96%), chlorpyrifos (17.20 and 15.97%), and dimethoate (17.78 and 15.81%). The coefficient of variation values were—emamectin benzoate (17.74 and 5.44%), lambda-cyhalothrin (21.15 and 5.16%), imidacloprid (13.01 and 5.65%), chlorpyrifos (16.72 and 4.85%), and dimethoate (28.36 and 7.93%). The results of the study show that the quantitative spraying method is more effective than the leaf-dipping method in precision and reproducibility. The results of this study will offer a useful reference for selecting a more scientific and rational method for pesticide risk assessment.

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Yanyan Chi, Kang Qiao, Hui Jiang, Ronghua Lin, and Kaiyun Wang "Comparison of Two Acute Toxicity Test Methods for the Silkworm (Lepidoptera: Bombycidae)," Journal of Economic Entomology 108(1), 145-149, (1 February 2015). https://doi.org/10.1093/jee/tou016
Received: 28 July 2014; Accepted: 30 September 2014; Published: 1 February 2015
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