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1 October 2015 Effects of X-Ray Irradiation on Male Navel Orangeworm Moths (Lepidoptera: Pyralidae) on Mating, Fecundity, Fertility, and Inherited Sterility
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Abstract

Male adult navel orangeworm, Amyelois transitella (Walker), were irradiated using a laboratory scale x-ray irradiation unit to determine the required dose for complete egg sterility of mated female moths and inherited sterility of F1 and F2 generations. Adult male A. transitella were irradiated in two separate experiments at 100–300Gy and 50–175Gy. Mating frequency, fecundity, and fertility of normal females crossed with irradiated parental males was compared with the mating of nonirradiated moths. Mating frequency was 100% for females crossed with nonirradiated control males. At male treatment doses of ≥150Gy the percentage of females found unmated increased, while multiple-mated females decreased. Female fecundity was not affected while fertility was affected in a dose-dependent relationship to exposure of parental males to x-ray irradiation. Embryonic development of eggs to the prehatch stage and egg eclosion did not occur at radiation doses ≥125Gy. Emergence of F1 adults was low and occurred only for progeny of parental males exposed to doses ≤100Gy, with no emergence at ≥125Gy. Though fecundity appeared similar for control and irradiated F1 females, no F2 eggs hatched for the test exposures of 50–100Gy. Based on our results, a dose of ≥125Gy had efficacy in inducing both primary parental sterility in treated male moths and inherited sterility in F1 male and female moths. Results suggest that A. transitella might be considered a candidate for the sterile insect technique using adults irradiated at these relatively low x-ray exposure doses.

Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.
Douglas M. Light, Inna Ovchinnikova, Eric S. Jackson, and Ronald P. Haff "Effects of X-Ray Irradiation on Male Navel Orangeworm Moths (Lepidoptera: Pyralidae) on Mating, Fecundity, Fertility, and Inherited Sterility," Journal of Economic Entomology 108(5), 2200-2212, (1 October 2015). https://doi.org/10.1093/jee/tov201
Received: 11 June 2015; Accepted: 19 June 2015; Published: 1 October 2015
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