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1 June 2005 Correlation between Fenvalerate Resistance and Cytochrome P450-mediated O-Demethylation Activity in Helicoverpa armigera (Lepidoptera: Noctuidae)
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Abstract

Cytochrome P450 monooxygenases are a major metabolic mechanism responsible for pyrethroid resistance in Helicoverpa armigera (Hübner) from Asia. Cytochrome P450-mediated O-demethylation activity toward p-nitroanisole (PNOD) of individual fourth instars was determined in five strains of H. armigera by using a microplate reader. The four resistant strains of YS, HD, YGF, and YG59 had 6-, 71-, 2,540-, and 11,800-fold resistance, respectively, to fenvalerate in comparison with the susceptible BK77 strain. Their mean PNOD activity was 4-, 10-, 24-, and 60-fold, respectively, compared with the BK77 strain. A strong positive correlation (correlation coefficient r = 0.98) between PNOD activity and fenvalerate resistance was found. Of 48 larvae from each strain, only 4% larvae of the susceptible BK77 strain had detectable PNOD activity, whereas 25, 33, 79, and 96% of larvae from the resistant strains YS, HD, YGF, and YG59 exhibited PNOD activity, respectively. There was a clear discrimination of patterns of PNOD frequency distribution between H. armigera strains and their magnitudes of fenvalerate resistance. The PNOD activity can be used as a biochemical marker for monooxygenase-mediated pyrethroid resistance in field populations of H. armigera.

Song Chen, Yihua Yang, and Yidong Wu "Correlation between Fenvalerate Resistance and Cytochrome P450-mediated O-Demethylation Activity in Helicoverpa armigera (Lepidoptera: Noctuidae)," Journal of Economic Entomology 98(3), 943-946, (1 June 2005). https://doi.org/10.1603/0022-0493-98.3.943
Received: 17 July 2004; Accepted: 1 December 2004; Published: 1 June 2005
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