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1 February 2006 Identification of Two Subterranean Termite Species (Isoptera: Rhinotermitidae) using Cellulase Genes
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Abstract

To identify subterranean termite species, we designed a pair of common primers that amplified 381-bp fragments from cDNAs encoding the endo-β-1,4-glucanases (EGases) of Coptotermes formosanus Shiraki and Reticulitermes speratus (Kolbe). cDNAs from C. formosanus and R. speratus, and genomic DNA from R. speratus, were amplified by polymerase chain reaction (PCR) by using this primer pair and then cloned and sequenced. Sequences amplified from C. formosanus cDNA displayed 97–99% identity to cDNA encoding the EGase of C. formosanus (CfEG), and 92–94% identity to cDNA encoding the EGase of R. speratus (RsEG). By contrast, cDNA from R. speratus displayed 99–100% identity to RsEG cDNA and 93–94% identity to CfEG cDNA. CfEG and RsEG cDNAs can therefore be used as markers for the identification of these termite species. This is the first report of the successful identification of termite species by using cDNA and genomic DNA sequences of termite origin.

Shuji Itakura, Takafumi Masuta, Hiromi Tanaka, and Akio Enoki "Identification of Two Subterranean Termite Species (Isoptera: Rhinotermitidae) using Cellulase Genes," Journal of Economic Entomology 99(1), 123-128, (1 February 2006). https://doi.org/10.1603/0022-0493(2006)099[0123:IOTSTS]2.0.CO;2
Received: 18 November 2004; Accepted: 15 September 2005; Published: 1 February 2006
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