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1 July 2012 Field Evaluation of a Kudzu/Cottonseed Oil Formulation on the Persistence of the Beet Armyworm Nucleopolyhedrovirus
Martin Shapiro, Said El Salamouny, D. Michael Jackson, B. Merle Shepard
Author Affiliations +
Abstract

A plant extract from kudzu, Pueraria lobata (Willd.) Ohwi (Fabaceae), was tested as a UV protectant for the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), nucleopolyhedrovirus (SeMNPV), with and without the addition of an oil/emulsifier (cottonseed oil/lecithin) formulation. Aqueous and oil emulsion formulations of SeMNPV were applied to collards and the residual virus activity was determined for 7 d. Sunlight exposure of SeMNPV resulted in an activity loss of 42%, 85%, and 95% at days 2, 4, and 7, respectively. The addition of the oil/emulsifier to SeMNPV did not provide UV protection. At days 2, 4, and 7, activity losses were 67%, 84%, and 92%, respectively. Whereas the addition of kudzu (5%) to SeMNPV provided significant UV protection during the sunlight exposure period, activity losses of 17%, 62%, and 76% occurred at days 2, 4, and 7, respectively. The greatest UV protection for SeMNPV was achieved when cottonseed oil/lecithin were used in conjunction with kudzu. In this formulation, activity losses were 2%, 40%, and 55% at days 2, 4, and 7, respectively. Although the mode of action is currently unknown, the addition of cottonseed oil to kudzu resulted in an increase of both UVB (280 - 320 nm) and UVA (320 - 400 nm) absorbance.

Martin Shapiro, Said El Salamouny, D. Michael Jackson, and B. Merle Shepard "Field Evaluation of a Kudzu/Cottonseed Oil Formulation on the Persistence of the Beet Armyworm Nucleopolyhedrovirus," Journal of Entomological Science 47(3), 197-207, (1 July 2012). https://doi.org/10.18474/0749-8004-47.3.197
Received: 15 September 2011; Accepted: 1 December 2011; Published: 1 July 2012
KEYWORDS
kudzu/cottonseed formulation
persistence
Spodoptera exigua
UV protectant
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