A survey of twospotted spider mite, Tetranychus urticae Koch, hosts was conducted during the spring months of 2007, 2008, and 2009 in Mississippi. Uncultivated field borders that supported a diverse flora of herbaceous plants and adjacent in-field weeds were sampled from late-March through May for the presence of twospotted spider mite. Twospotted spider mite preference for each plant species was determined and ranked in a 0 - 3 scale. Henbit, Lamium amplexicaule L., was the most consistent and preferred host of twospotted spider mite in Mississippi. Carolina geranium, Geranium carolinianum L., cutleaf geranium, Geranium dissectum L., vetch, Vicia spp., volunteer soybean, Glycine max L., purple deadnettle, Lamium purpureum L., and spiny sowthistle, Sonchus asper (L.) Hill, were other frequently infested dicotyledonous species. Of the monocotyledonous species sampled, only rescuegrass, Bromus catharticus Vahl, john-songrass, Sorghum halepense (L.) Pers., and volunteer corn, Zea mays L., appeared to be major hosts of twospotted spider mite during spring months. Italian ryegrass, Lolium perenne (L.) spp. multiflorum (Lam.), did not appear to be an important host of twospotted spider mite at any location, which is a benefit considering glyphosate resistant biotypes are very common in many Mississippi fields. Equally important, twospotted spider mite was not found on annual bluegrass, Poa annua L., which occurs in all Mississippi fields during winter and spring. The host list generated from this study can be directly used to refine early-season twospotted spider mite management, and also help support future research.