Human-wildlife conflict (HWC) is a widespread issue of increasing concern to conservationists, as it impacts people's lives and livelihoods and reduces their tolerance to the species concerned. HWC is often interpreted as a result of people encroaching upon and destroying natural habitats, but some incidents could be linked to economically driven emigration that results in depopulation and institutional and cultural disruption. Here we use an ethnobiological approach to gain insights on HWC dynamics from a case study in Mexico, where emigration is common in rural areas. We carried out a five-year study of HWC in the Sierra Gorda Biosphere Reserve, a biodiversity-rich protected area in Mexico that also supports a human population of nearly 100,000 mostly poor and marginalized people. We found that villagers reported conflict incidents involving 25 terrestrial vertebrate species, contradicting the original self-perception that HWC mostly involved cattle ranchers and a few large carnivore species. As a response, we develop a multi-layered assessment of villagers' perspectives, emotions, and attitudes towards wildlife based on the local roles of gender, probability of encountering wildlife, and the conflicting moral beliefs and switching ethical responses of people with different cultural and economic backgrounds. Our assessment identifies the need for pluralistic approaches to enhance the sustainable use and management of wildlife in the Sierra Gorda Biosphere Reserve.