In South Africa, particularly Gauteng Province, populations of the large, explosive-breeding Giant Bullfrog (Pyxicephalus adspersus) are suffering increasing habitat loss due to encroaching urbanization. To investigate the spatial habitat requirements of this regionally threatened species, 70 adult frogs were radio- or spool-tracked during five summers around a periurban breeding site. Male and female P. adspersus moved a maximum overnight distance of 350 m when returning to their burrows postspawning. On average, animals of either sex used one long-term burrow (LTB) in a summer. Four males each used a single LTB or burrowing area for two or three consecutive summers. The LTBs of females were situated almost 4 times further (mean  =  446.8 m) from the seasonal dams where spawning occurred than those of males (mean  =  131.0 m). Female body condition was significantly positively correlated with distance of their burrows from the seasonal dams (r_s  =  0.77). Limited evidence indicated that adult P. adspersus probably forage mostly within 20 m of their burrows. To protect the LTBs of all radiotracked animals a 950-1,000-m wide buffer would be necessary around the seasonal dams. Because adult P. adspersus seem to be philopatric, juvenile dispersal is predicted to be largely responsible for gene flow among populations.