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1 January 2015 Phylogenetics Based on Partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
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Abstract

The only virus sequenced and studied in triatomines is the Triatoma virus, from the Dicistroviridae family, which causes delayed development, reduced oviposition, and premature death of infected insects. With the goal of expanding the sequences already obtained in previous years and verifying if any changes occurred in their genomic sequences, 68 samples of triatomines from several provinces of Argentina were analyzed. Sixteen positive samples were obtained by Reverse Transcription (RT)-polymerase chain reaction using the VP3-VP1 subregion of open reading frame-2 as a diagnostic method; after sequencing, 11 samples were obtained from Triatoma infestans. These new sequences showed no significant differences in the analyzed regions, which were not grouped by species or habitat or geographical distribution. There were no differences when compared with the sequences found during 2002–2012, all obtained from the wild.We conclude that despite being an RNA virus, the different sequences show high homology.

© The Author 2015. Published by Oxford University Press on behalf of the Entomological Society of America.
María Laura Susevich, Gerardo Aníbal Marti, Agustín Balsalobre, and María Gabriela Echeverría "Phylogenetics Based on Partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats," Journal of Insect Science 15(1), 1-4, (1 January 2015). https://doi.org/10.1093/jisesa/ieu139
Received: 29 October 2013; Accepted: 29 January 2014; Published: 1 January 2015
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