Translator Disclaimer
1 July 2000 Development and Evaluation of a Dipstick Assay for Detection ofPlasmodium falciparumandP. vivaxsporozoites in Mosquitoes (Diptera: Culicidae)
Author Affiliations +
Abstract

We developed a nitrocellulose-based, dipstick circumsporozoite (CS)-enzyme immunoassay [ELISA] for the simultaneous detection ofPlasmodium falciparumandP. vivax−210 CS protein. The assay had a detection threshold of <250P. falciparumor 400P. vivaxsporozoites per sample, gave results concordant with dissection of salivary glands and CS-ELISA, but was slightly less sensitive than the CS-ELISA in microtiter plates. The assay consistently detected one infected mosquito in a pool of 10 or 20 mosquitoes, and was 100% specific in discriminating between species ofPlasmodiumwhen mosquito suspensions were spiked with sporozoites. The assay could be completed in 1 h, required no specialized equipment, and therefore was useful for field applications.

Russell E. Coleman, James F. Barth, Michael J. Turell, Scott W. Gordon, Jetsummon Sattabongkot, Robert Copeland, and Robert A. Wirtz "Development and Evaluation of a Dipstick Assay for Detection ofPlasmodium falciparumandP. vivaxsporozoites in Mosquitoes (Diptera: Culicidae)," Journal of Medical Entomology 37(4), (1 July 2000). https://doi.org/10.1603/0022-2585-37.4.581
Received: 23 March 1999; Accepted: 1 March 2000; Published: 1 July 2000
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top