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1 July 2001 Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR
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Abstract

Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN-4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%).

Chung Youne Kow, Lim Loo Koon, and Pang Fung Yin "Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR," Journal of Medical Entomology 38(4), (1 July 2001). https://doi.org/10.1603/0022-2585-38.4.475
Received: 25 April 2000; Accepted: 1 November 2000; Published: 1 July 2001
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