Translator Disclaimer
1 March 2002 Glass Capillary Tube Feeding: A Method for Infecting Nymphal Ixodes scapularis (Acari: Ixodidae) with The Lyme Disease Spirochete Borrelia burgdorferi
Author Affiliations +
Abstract

We evaluated an artificial capillary feeding method to infect nymphal Ixodes scapularis (Say) ticks with Borrelia burgdorferi, the causative agent of Lyme disease. Thirty to 70% of the nymphs were infected after feeding for 2.5 h from glass capillary tubes filled with a solution of spirochetes. Capillary infection was stable and persisted in the nymphs for at least 10 d after feeding. Capillary feeding also maintained natural vector competence patterns because I. scapularis ticks acquired infection unlike Dermacentor variablis (Say), which did not become infected. Capillary infected I. scapularis nymphs were capable of transmitting the infection to naive mice although not as efficiently as naturally infected nymphs. The capillary infection method is convenient and is a better alternative to syringe inoculation as a means of infecting animals with B. burgdorferi.

Anne H. Broadwater, Daniel E. Sonenshine, Wayne L. Hynes, Shane Ceraul, and Aravinda M. de Silva "Glass Capillary Tube Feeding: A Method for Infecting Nymphal Ixodes scapularis (Acari: Ixodidae) with The Lyme Disease Spirochete Borrelia burgdorferi," Journal of Medical Entomology 39(2), (1 March 2002). https://doi.org/10.1603/0022-2585-39.2.285
Received: 28 February 2001; Accepted: 1 October 2001; Published: 1 March 2002
JOURNAL ARTICLE
8 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top