The surfaces of the body segments and appendages of male and female Dermatobia hominis (L. Jr., 1781) were studied by scanning electron microscopy with the objective of contributing to the current knowledge of the morphology of this insect. The image analyses were done with consideration of the scientific literature on morphology, ultrastructure, and insect sensory structures. A new criterion for distinguishing the sexes of this fly was observed as well as a new setiform empodium type associated with other smaller setae. Different types of trichoid sensilla were observed, as well as spinules, a type of cuticular setiform projection and a scale-like structure. The possible function of the sensilla was discussed, and sensilla with mechanosensory and chemosensory functions were found. Pilose regions that could be involved in the production and/or liberation of pheromones were encountered in the intersegmental spaces of the female abdomen. The current study of the ultrastructure of the body surface of D. hominis contributes to current knowledge of morphology, taxonomy, and sensory structures of this species.