Translator Disclaimer
1 November 2004 An In Vitro Larval Immersion Microassay for Identifying and Characterizing Candidate Acaricides
Author Affiliations +
Abstract

We have optimized a larval immersion microassay (LIM) that offers superior sensitivity, flexibility to accommodate multiple formulations, and a robust capability for rapidly screening many compounds with a minimal requirement of test article for evaluation. Dose–response studies were conducted for representative members from the organophosphate, pyrethroid, pyrazole, carbamate, macrocyclic lactone, and formamidine chemistries against Amblyomma americanum (L.). Time–response experiments revealed that permethrin was the most rapid acting, whereas fipronil had the slowest speed-of-kill against A. americanum. Comparison of drug susceptibility profiles between multiple ixodid ticks suggests that A. americanum is an effective model for predicting compound potency against Boophilus spp. in this bioassay. The LIM is suitable for the identification and characterization of active molecules from small- and medium-sized compound or natural product libraries, and it can be a useful tool to prioritize molecules for further in vivo testing in animal models.

W. Hunter White, Philip R. Plummer, Connie J. Kemper, Robert J. Miller, Ronald B. Davey, David H. Kemp, Suzanne Hughes, Charles K. Smith, and Jesus A. Gutierrez "An In Vitro Larval Immersion Microassay for Identifying and Characterizing Candidate Acaricides," Journal of Medical Entomology 41(6), 1034-1042, (1 November 2004). https://doi.org/10.1603/0022-2585-41.6.1034
Received: 25 June 2004; Accepted: 1 August 2004; Published: 1 November 2004
JOURNAL ARTICLE
9 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top