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1 March 2006 Use of Enzyme-Linked Immunosorbent Assay for Detection of Natural Leishmania Infections in Phlebotomine Sand Flies from Southeastern Turkey
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Abstract

In total, 320 phelebotomine sand flies from Alibozlu (Osmaniye), Kizyusuflu (Osmaniye), and Sanliurfa in southeastern Turkey were tested for the detection and identification of Leishmania in vector sand flies by enzyme-linked immunosorbent assay with species-specific monoclonal antibodies. We used monoclonal antibodies that recognize both Leishmania tropica and Leishmania major, and a monoclonal antibody specific only to L. tropica. Phosphate-buffered saline and monoclonal antibody M2 recognizing Leishmania amazonensis were used as controls. Infection rates of sand flies were 0.9% in Alibozlu, 0% Kizyusuflu, and 3.6% in Sanliurfa. Positive sand flies were identified as Phlebotomus sergenti Parrot and Phlebotomus major syriacus Adler & Theodor.

Ayfer Pazarbasi, Davut Alptekin, H. Umit Luleyap, Mulkiye Kasap, and Halil Kasap "Use of Enzyme-Linked Immunosorbent Assay for Detection of Natural Leishmania Infections in Phlebotomine Sand Flies from Southeastern Turkey," Journal of Medical Entomology 43(2), (1 March 2006). https://doi.org/10.1603/0022-2585(2006)043[0248:UOEIAF]2.0.CO;2
Received: 24 January 2005; Accepted: 14 November 2005; Published: 1 March 2006
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