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1 May 2009 Inhibitor of Cysteine Peptidase Does Not Influence the Development of Leishmania mexicana in Lutzomyia longipalpis
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Abstract

It has been proposed that the natural cysteine peptidase inhibitor ICP of Leishmania mexicana protects the protozoan parasite from insect host proteolytic enzymes, thereby promoting survival. To test this hypothesis, L. mexicana mutants deficient in ICP were evaluated for their ability to develop in the sand fly Lutzomyia longipalpis. No significant differences were found between the wild-type parasites, two independently derived ICP-deficient mutants, or mutants overexpressing ICP; all lines developed similarly in the sand fly midgut and produced heavy late-stage infections. In addition, recombinant L. mexicana ICP did not inhibit peptidase activity of the midgut extracts in vitro. We conclude that ICP has no major role in promoting survival of L. mexicana in the vectorial part of its life cycle in L. longipalpis.

©2009 Entomological Society of America
Lucie Jecna, Anna Svarovska, Sebastien Besteiro, Jeremy C. Mottram, Graham H. Coombs, and Petr Volf "Inhibitor of Cysteine Peptidase Does Not Influence the Development of Leishmania mexicana in Lutzomyia longipalpis," Journal of Medical Entomology 46(3), (1 May 2009). https://doi.org/10.1603/033.046.0327
Received: 18 June 2008; Accepted: 1 November 2008; Published: 1 May 2009
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