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1 May 2010 The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito, Ochlerotatus triseriatus
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Abstract

Saliva of blood-sucking arthropods contains a complex mixture of peptides that affect their host's hemostasis, inflammation, and immunity. These activities can also modify the site of pathogen delivery and increase disease transmission. Saliva also induces hosts to mount an antisaliva immune response that can lead to skin allergies or even anaphylaxis. Accordingly, knowledge of the salivary repertoire, or sialome, of a mosquito is useful to provide a knowledge platform to mine for novel pharmacological activities, to develop novel vaccine targets for vector-borne diseases, and to develop epidemiological markers of vector exposure and candidate desensitization vaccines. The mosquito Ochlerotatus triseriatus is a vector of La Crosse virus and produces allergy in humans. In this work, a total of 1,575 clones randomly selected from an adult female O. triseriatus salivary gland cDNA library was sequenced and used to assemble a database that yielded 731 clusters of related sequences, 560 of which were singletons. Primer extension experiments were performed in selected clones to further extend sequence coverage, allowing for the identification of 159 protein sequences, 66 of which code for putative secreted proteins. Supplemental spreadsheets containing these data are available at  http://exon.niaid.nih.gov/transcriptome/Ochlerotatus_triseriatus/S1/Ot-S1.xls and  http://exon.niaid.nih.gov/transcriptome/Ochlerotatus_triseriatus/S2/Ot-S2.xls.

© 2010 Entomological Society of America
Eric Calvo, Irma Sanchez-Vargas, Michalis Kotsyfakis, Amanda J. Favreau, Kent D. Barbian, Van M. Pham, Kenneth E. Olson, and José M. C. Ribeiro "The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito, Ochlerotatus triseriatus," Journal of Medical Entomology 47(3), (1 May 2010). https://doi.org/10.1603/ME09226
Received: 4 September 2009; Accepted: 1 March 2010; Published: 1 May 2010
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