Translator Disclaimer
1 September 2010 Development of an Enzyme-Linked Immunosorbent Assay to Identify Host-Feeding Preferences of Phlebotomus Species (Diptera: Psychodidae) in Endemic Foci of Visceral Leishmaniasis in Nepal
Author Affiliations +
Abstract

Anthroponotic visceral leishmaniasis, transmitted by Phlebotomus argentipes Annandale & Brunetti (Diptera: Psychodidae) sand flies, is regarded as a major problem of public health importance in the Indian subcontinent. Understanding the feeding behavior of the vector can be used to investigate changes in human-vector contact during intervention programs. An enzyme-linked immunosorbent assay (ELISA) was modified to make it suitable to identify the origin of P. argentipes and Phlebotomus papatasi Scopoli (Diptera: Psychodidae) blood meals. The sensitivity and specificity of the precipitin ring test and ELISA were compared, as well as the stability of the tests across different stages of blood meal digestion. The ELISA was more sensitive and specific than the precipitin test for identifying the sources of blood meals. When using the ELISA method with a plate reader, it was possible to obtain 100% sensitivity and specificity. When comparing the techniques across digestion stages, it was found that there was a drop in sensitivity, 48 and 72 h postblood meal for precipitin and visually read ELISA, respectively. However, the sensitivity of the ELISA using a plate reader was not altered by the digestion time. The feeding habits of P. argentipes and P. papatasi from the Terai region of Nepal, determined by the ELISA developed, showed P. papatasi to be highly anthropophilic, and P. argentipes appeared to feed both on humans and animals, in particular bovines.

© 2010 Entomological Society of America
Ian Burniston, Lalita Roy, Albert Picado, Murari Das, Suman Rijal, Matthew Rogers, Marc Coosemans, Marleen Boelaert, Clive Davies, and Mary Cameron "Development of an Enzyme-Linked Immunosorbent Assay to Identify Host-Feeding Preferences of Phlebotomus Species (Diptera: Psychodidae) in Endemic Foci of Visceral Leishmaniasis in Nepal," Journal of Medical Entomology 47(5), 902-906, (1 September 2010). https://doi.org/10.1603/ME09184
Received: 20 July 2009; Accepted: 1 April 2010; Published: 1 September 2010
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top