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1 January 2011 Host Blood Meal Identification by Multiplex Polymerase Chain Reaction for Dispersal Evidence of Stable Flies (Diptera: Muscidae) between Livestock Facilities
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Abstract

A species-specific multiplex polymerase chain reaction targeting the cytochrome b gene of cattle, horses, humans, and dogs was developed to determine the blood meal sources of stable flies, Stomoxys calcitrans (L.), collected from Florida equine facilities. Of 595 presumptive blood-fed stable flies analyzed, successful host amplification was obtained in 350, for a field host-detection efficiency of 58.8%. The majority of analyzed stable flies had fed on cattle (64.6%), followed by horses (24.3%), humans (9.5%), and dogs (1.6%). A survey of animal-enclosed pastures occurring within 3 km of stable fly collection sites revealed that the nearest cattle were between 0.8 and 1.5 km from the four horse farm sampling sites. Cattle-feeding frequencies were greater on farms where cattle were located at distances of 0.8 km, suggesting that between farm differences in host-feeding frequency is related to the number of and distance from a particular host type. Time course evaluations of previously laboratory-fed stable flies demonstrated that host-detection efficiency with this system was 100, 50, and 0% when flies were evaluated at 16, 24, and 48 h postblood feeding, respectively. The results of this study suggest short-term stable fly dispersal of up to 1.5 km in a 48-h time period. The implications of these findings are discussed.

© 2011 Entomological Society of America
Jimmy B. Pitzer, Phillip E. Kaufman, Saundra H. Tenbroeck, and James E. Maruniak "Host Blood Meal Identification by Multiplex Polymerase Chain Reaction for Dispersal Evidence of Stable Flies (Diptera: Muscidae) between Livestock Facilities," Journal of Medical Entomology 48(1), 53-60, (1 January 2011). https://doi.org/10.1603/ME10123
Received: 12 May 2010; Accepted: 1 September 2010; Published: 1 January 2011
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