A naturally occurring mutation was detected within the probe binding region targeting the envelope gene sequence of West Nile virus used in real-time polymerase chain reaction assays to test mosquito pools and other samples. A single C→T transition 6nt from the 5′ end of the 16mer in the envelope gene probe-binding region at genomic position 1,194 reduced assay sensitivity. The mutation first was detected in 2009 and persisted at a low prevalence into 2011. The mutation caused a 0.4% false negative error rate during 2011. These data emphasized the importance of confirmational testing and redundancy in surveillance systems relying on highly specific nucleic acid detection platforms.
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1 July 2012
A Naturally Occurring Mutation Within the Probe-Binding Region Compromises a Molecular-Based West Nile Virus Surveillance Assay for Mosquito Pools (Diptera: Culicidae)
Aaron C. Brault,
Ying Fang,
Maureen Dannen,
Michael Anishchenko,
William K. Reisen
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Journal of Medical Entomology
Vol. 49 • No. 4
July 2012
Vol. 49 • No. 4
July 2012
mosquito pool
mutation
probe binding region
qRT-PCR
surveillance