Translator Disclaimer
1 January 2013 The Group 10 Allergen of Dermatophagoides farinae (Acari: Pyroglyphidae): cDNA Cloning, Sequence Analysis, and Expression in Escherichia coli BL21
Author Affiliations +
Abstract

Dermatophagoides farinae Hughes, American house dust mite, is highly allergenic, producing symptoms in people worldwide. Identifying and cloning the allergens in this species may enable better diagnostic and therapeutic approaches. Here, we cloned, sequenced, and expressed the full-length cDNA encoding D. farinae group 10 allergen (Der f 10) isolated from dust mites in China. Bioinformatic analysis indicated that the 888 bp sequence encoded a cytoskeleton protein 295 amino acids long, with a molecular weight of ≈34 kDa. Sequence alignment with the group 10 allergens of Pyroglyphidae, Acaridae, and Glycyphagidae families revealed that the group 10 allergen from D. farinae is 95% similar to D. pteronyssinus Trouessart and Psoroptes ovis (Hering). These findings lay the groundwork for future studies, including large-scale production of recombinant Der f 10 allergen for diagnostic and therapeutic agents.

© 2013 Entomological Society of America
Yubao Cui, Ying Zhou, Yungang Wang, Guifang Ma, and Li Yang "The Group 10 Allergen of Dermatophagoides farinae (Acari: Pyroglyphidae): cDNA Cloning, Sequence Analysis, and Expression in Escherichia coli BL21," Journal of Medical Entomology 50(1), 205-208, (1 January 2013). https://doi.org/10.1603/ME12019
Received: 2 February 2012; Accepted: 1 July 2012; Published: 1 January 2013
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top